Journal: Electrophoresis

Article Title: A comparative study of CE-SDS, SDS-PAGE, and Simple Western: Influences of sample preparation on molecular weight determination of proteins.

doi: 10.1002/elps.202000199

Figure Lengend Snippet: Figure 1. Linear regression plots based on the logarithm of the MW versus the relative migration distance (Rf) respectively the reciprocal relative migration time (RMT) of MW marker proteins (Precision Plus ProteinTM Standard from Bio-Rad). (A) Linear regression of the data points while all points included on SDS-PAGE; (B) Linear regression of the data points, deviating points excluded on SDS-PAGE; (C) Residual plot of the data points out of (A); (D) Residual plot of the data points out of (B); (E) Linear regression of the data points while all points included on CE-SDS; (F) Linear regression of the data points, deviating points excluded on CE-SDS

Article Snippet: Molecular Weight Markers Maurice CE-SDS (ProteinSimple, a Bio-Techne Brand, San Jose, CA, USA, specified size range: 10–270 kDa) and ProteomeLabTM MW Sizing Standard (AB Sciex Germany GmbH, Darmstadt, Germany, specified size range: 10–225 kDa) were chosen as typical CE-SDS markers and Precision Plus ProteinTM Standard unstained (Bio-Rad Laboratories GmbH, Feldkirchen, Germany, specified size range: 10–250 kDa), Unstained Protein Standard (New England Biolabs GmbH, Frankfurt am Main, Germany, specified size range: 10–200 kDa) and BenchmarkTM Unstained Protein Ladder (Novex by life technologies, Thermo Fisher Scientific, MA, USA, specified size range: 10– 220 kDa) were chosen as typical SDS-PAGE markers.

Techniques: Migration, Marker, SDS Page

Journal: Electrophoresis

Article Title: A comparative study of CE-SDS, SDS-PAGE, and Simple Western: Influences of sample preparation on molecular weight determination of proteins.

doi: 10.1002/elps.202000199

Figure Lengend Snippet: Figure 2. Comparison of MW markers of five different man- ufacturers by 10% SDS-PAGE. The relevant lanes of the gel were reconstructed for better illustration using ChemDraw 19.0.1.28 (PerkinElmer Infor- matics, Inc.); a photo of the gel is shown in Figure S2 in the Supporting Information. Bio-Rad = Precision Plus ProteinTM Standard unstained by Bio-Rad; ProteinSim- ple = Molecular Weight Marker Maurice CE-SDS by ProteinSimple; Sciex = ProteomeLabTM MW Sizing Standard by Sciex; Biolabs = Unstained Protein Standard by New England Biolabs; Benchmark = BenchmarkTM

Article Snippet: Molecular Weight Markers Maurice CE-SDS (ProteinSimple, a Bio-Techne Brand, San Jose, CA, USA, specified size range: 10–270 kDa) and ProteomeLabTM MW Sizing Standard (AB Sciex Germany GmbH, Darmstadt, Germany, specified size range: 10–225 kDa) were chosen as typical CE-SDS markers and Precision Plus ProteinTM Standard unstained (Bio-Rad Laboratories GmbH, Feldkirchen, Germany, specified size range: 10–250 kDa), Unstained Protein Standard (New England Biolabs GmbH, Frankfurt am Main, Germany, specified size range: 10–200 kDa) and BenchmarkTM Unstained Protein Ladder (Novex by life technologies, Thermo Fisher Scientific, MA, USA, specified size range: 10– 220 kDa) were chosen as typical SDS-PAGE markers.

Techniques: Comparison, SDS Page, Molecular Weight, Marker